A simple, precise, accurate, reproducible and robust HPLC method was developed for the estimation of atazanavir and cobicistat in synthetic mixture. The method was validated as per ICH guidelines. Analysis of the drug was performed on Phenomenex Luna C18 (250 mm × 4.6 mm, 5 µ) column, in an isocratic mode employing pH 4.5 phosphate buffer: Acetonitrile (60:40 % v/v) as the mobile phase. UV-Visible detector at 238 nm was found to be suitable for detection. The method shows good peak shape, minimal tailing, with retention time 2.69 min for atazanavir and 4.79 min for cobicistat. Linearity was observed in the range of 15-45 µg/ml (r2 = 0.999) for atazanavir and 7.5-22.5 µg/ml (r2 = 0.999) for cobicistat. Recovery values were found to be 99.75 - 101.94 % and 99.33 – 101.88 % for atazanavir and cobicistat respectively. LOD of the method were found to be 5.06 µg/ml for atazanavir and 2.80 µg/ml for cobicistat. LOQ of the method were found to be 15.35 µg/ml and 8.50 µg/ml for atazanavir and cobicistat respectively. The statistical parameters were found within range.
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